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Registros recuperados: 115
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Qualification zoo-sanitaire du naissain de captage 2013 de l'huître creuse Crassostrea gigas (QUALIF) - Détection précoce des maladies en épreuve thermique de laboratoire ArchiMer
Petton, Bruno; Riobe, Marion; Koechlin, Hugo; Queau, Isabelle; Mingant, Christian; Ratiskol, Dominique; Le Grand, Jacqueline; Petton, Sebastien; Thomas, Yoann; Alunno-bruscia, Marianne; Pouvreau, Stephane; Pernet, Fabrice; Maurer, Daniele; D'Amico, Florence; Lagarde, Franck; Mortreux, Serge; Roque D'Orbcastel, Emmanuelle; Mille, Dominique; Geay, A.; Bouquet, A.-l.; Cochet, Helene.
Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Des travaux de qualification zoo-sanitaire menés depuis 2010 ont montré qu’un lot de naissains issus du captage naturel sur deux était infecté par le virus bien avant l’apparition des mortalités dans le milieu naturel, mais qu’a contrario les naissains d’écloserie expertisés présentaient un moindre risque de contamination. Dans le contexte de crise sanitaire chronique, l’objectif de notre étude était d’ obtenir une information qualitative relative au statut zoo-sanitaire OsHV -1 μ V ar des...
Tipo: Text Palavras-chave: Huitre creuse; OsHV - 1μVar; Epreuve thermique.; Maladie; Naissain; Pacific oyster; Spat; Disease; Thermal challenge; OsHV-1 μVar.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00276/38682/37180.pdf
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Recherche de gisements de captage naturel de Crassostrea gigas en lagune de Thau.Répartition spatiotemporelle du naissain, aspects culturaux et économiques. ArchiMer
Lagarde, Franck; Perignon, Adeline; Gervasoni, Erika; Mortreux, Serge; Meddah, Slem; Leurion, Axel; Le Gall, Patrik; Guillou, Jean-louis; Boj, Marie; Fuhrmann, Marine; Richard, Marion; Ubertini, Martin; Fiandrino, Annie; Roque D'Orbcastel, Emmanuelle; Pouvreau, Stephane.
Le travail est axé sur la volonté de la filière conchylicole méditerranéenne pour s'approvisionner en naissains d'huîtres creuses sauvages d’origine lagunaire. L'observatoire du captage naturel, mis en place entre 2012 et 2014, a permis de découvrir des gisements de naissains collectés naturellement dans la lagune de Thau selon un protocole défini à l’échelle expérimentale. Bien que très hétérogènes spatialement et temporellement, les densités de naissains sur les collecteurs révèlent des catégories de captage allant de "moyen i. e. 20 à 200 naissains par coupelle" à "excellent i. e. 200 à 2000 naissains par coupelle " sur certaines stations expérimentales. Les expériences de captage naturel à échelle semi-industrielle (avec filière de coupelles en lagune)...
Tipo: Text Palavras-chave: Captage naturel; Crassostrea gigas; Recrutement; Conchyliculture méditerranéenne; Lagune..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00260/37103/35632.pdf
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Recruitment of the Pacific oyster Crassostrea gigas in a shellfish-exploited Mediterranean lagoon: discovery, driving factors and a favorable environmental window ArchiMer
Lagarde, Franck; Roque D'Orbcastel, Emmanuelle; Ubertini, Martin; Mortreux, Serge; Bernard, Ismael; Fiandrino, Annie; Chiantella, Claude; Bec, Beatrice; Roques, Cecile; Bonnet, Delphine; Miron, Gilles; Richard, Marion; Pouvreau, Stephane; Lett, Christophe.
In the context of increasing demand for environmental recovery, aquatic systems may face the challenge of evolving under oligotrophication. This is the case in Mediterranean lagoons, in particular the shellfish-farmed Thau lagoon in France, where we studied recruitment of the Pacific oyster Crassostrea gigas. Oyster spat and environmental parameters were monitored at several sampling sites for 3 yr (2012 to 2014) using an original method with a temporal overlap deployment of collectors to study pre- and post-settlement processes and to identify the best conditions for recruitment. Contrary to the ‘no Pacific oyster reproduction’ paradigm in Mediterranean lagoons, our study showed that recruitment of this introduced species is possible in the Thau lagoon at...
Tipo: Text Palavras-chave: Crassostrea gigas; Oyster spat; Pediveliger; Metamorphosis; Recruitment; Oligo trophication; Larval ecology.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00398/50899/51672.pdf
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Reproduction de l’huître creuse, Crassostrea gigas, en Baie de Bourgneuf : Mise en place d’un suivi en 2009 dans le cadre du projet national Velyger ArchiMer
Pouvreau, Stephane; Le Souchu, Pierrick; Breerette, Stephane; Claude, Serge; Langlade, Aime; Bouget, Jean-francois; Hitier, Benoist; Martin, Anne-genevieve; Bedier, Edouard.
Dans le cadre d’un réseau national (VELYGER), la Baie de Bourgneuf (partie Nord de la Baie) a fait l’objet en 2009 d’un suivi détaillé de la reproduction de l’huître creuse, Crassostrea gigas : gamétogenèse et ponte des adultes, abondance et développement larvaire ainsi qu’intensité et survie du captage sur collecteurs. Les résultats de ce suivi ont été mis en ligne, sous la forme de bulletin hebdomadaire, en temps quasi-réel sur le site : http://www.ifremer.fr/velyger. Il ressort, en 2009, que : (1) la gamétogenèse des huîtres s’est déroulée favorablement en permettant une ponte massive dans la première quinzaine de juillet, plus précoce mais aussi plus importante que sur des sites traditionnels (Arcachon et Marennes-Oléron) ; (2) les larves ont été...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12273/9064.pdf
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Spatial patterns in the condition index of the wild Pacific oyster Crassostrea gigas in a macrotidal coastal ecosystem: Influence of tidal processes and beyond ArchiMer
Gasmi, Sonia; Bernard, Ismael; Pouvreau, Stephane; Maurer, Daniele; Schaal, Gauthier; Ganthy, Florian; Cominassi, Louise; Allain, Gwenhael; Sautour, Benoit; David, Valerie.
In macrotidal coastal ecosystems, spatial heterogeneity of the water column properties is induced by both oceanic and continental influences. Hydrodynamic processes generate a land-sea gradient of environmental conditions, affecting the biological performances of sedentary organisms. The aim of the present study is to establish an extensive spatial assessment in the reproductive investment of the wild Pacific oyster Crassostrea gigas in Arcachon Bay. This is done by looking for a relationship between the Lawrence and Scott condition index (LSCI) and two tidal processes: the immersion level (IL) and the local oceanic flushing time (LoFt). The LSCI of C. gigas was assessed, just before gamete release, at 68 sampling stations in Arcachon Bay. Oyster...
Tipo: Text Palavras-chave: Reproductive investment; Crassostrea gigas; Macrotidal lagoon; Immersion level; Oceanic flushing; Environmental variability.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46803/46670.pdf
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Suivi national des mortalités au stade naissain d'un lot témoin et d'un lot à survie améliorée pour Crassostrea gigas ArchiMer
Degremont, Lionel; Maurouard, Elise; Nourry, Max; Seugnet, Jean-luc; Bedier, Edouard; Fleury, Elodie; Langlade, Aime; Pernet, Fabrice; Pouvreau, Stephane; Le Souchu, Pierrick; Normand, Julien; D'Amico, Florence; Rumebe, Myriam; Cantin, Christian; Barret, Jean; Le Gall, Patrik; Baud, Jean-pierre; Cochennec-laureau, Nathalie; Gervasoni, Erika; Pelissier, Pablo.
Ce rapport présente la comparaison des mortalités de deux lots de naissains de Crassostrea gigas dans plusieurs bassins conchylicoles et déterminer le gain de survie obtenu par la sélection pour des huîtres diploïdes
Tipo: Text Palavras-chave: Mortalités; Huître creuse; Crassostrea gigas; Sélection génétique; Naissain.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00065/17644/15180.pdf
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Synthèse PERLE. Programme d’Expérimentation et de recherche sur L’huître plate ostrea Edulis ArchiMer
Hussenot, Mathieu; Pouvreau, Stephane; Duchene, Julie; Freulon, Henry; Arzul, Isabelle; Lapegue, Sylvie.
En complément du rapport final (Anonyme (2014). PERLE. Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis. Rapport final de l'ensemble du projet 2011-2014. http://archimer.ifremer.fr/doc/00248/35932/), ce document présente une synthèse des principaux résultats du programme PERLE et ouvre de multiples perspectives pour les années à venir.
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00249/36060/34610.pdf
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Table ronde : les analyses biochimiques de PLG dans la chair des coquillages ArchiMer
Faury, Nicole; Geairon, Philippe; Moal, Jeanne; Pouvreau, Stephane; Razet, Daniel; Ropert, Michel; Soletchnik, Patrick.
Proximate biochemical analysis (proteins, lipids and carbohydrates) are performed on a regular basis by the various laboratories fom the Aquaculture Resources Directorate. Methods initially developed to analyse hydrological parameters were firstly transfered to assess meat components.1t appears now important to review and update the commonly used methods (Lowry et al., (1951), Dubois et al.,(l956), Marsh et Weinstein, (1966)), for our specifie purposes. A round table was organized to facilitate discussion among researchers and technicians from both Directorates 'Aquaculture Resources' and 'Product Valorization'and external partners, University of Caen. Several communications reviewing former and recent studies were done. Colleagues from the Directorate...
Tipo: Text Palavras-chave: Glycogen; Carbohydrates; Lipids; Proteins; Methods comparison; Proximate biochemical composition; Glycogène; Carbohydrates; Lipiedes; Protéines; Comparaison méthodes; Composition biochimique.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/rapport-6378.pdf
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Temperature and photoperiod drive Crassostrea gigas reproductive internal clock ArchiMer
Fabioux, Caroline; Huvet, Arnaud; Le Souchu, Pierrick; Le Pennec, Marcel; Pouvreau, Stephane.
This study examined the gametogenic cycle of Crassostrea gigas in controlled conditions over one year, with a focus on the initiation of gametogenesis. This work analysed also the role of temperature and photoperiod in the regulation of oyster reproduction. Broodstock were maintained in natural (NC), accelerated (AC) and perpetual winter (WC) conditions of temperature and photoperiod, with feeding ad libitum. Qualitative and quantitative analyses of the reproductive pattern were performed using biometric measurement approach, sex ratio determination, histology and a gonad filling index. Each experimental treatment led to different strategies for growth and resource allocation. The gametogenic cycle, appeared entirely modulated, accelerated or delayed, by...
Tipo: Text Palavras-chave: Temperature; Regulation; Gonad filling index; Gonial mitosis; Reproduction; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-826.pdf
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Temporal variability in the Pacific oyster spatfall along the French Coast: a new monitoring project ArchiMer
Pouvreau, Stephane; Maurer, Daniele; Auby, Isabelle; Soletchnik, Patrick; Mille, Dominique; Bernard, Ismael; Bedier, Edouard.
In France, spatfall of Pacific Oysters occurs traditionally in two shellfish area of the south west of France: the Marennes-Oléron bay and the Arcachon basin. The spat collected in both sites ensures approx. 70 % of the need of the French oysters production (the other part is produced in hatcheries). But, since ten years, recruitment have become highly variable especially in Arcachon basin. For instance, in this site, spatfall (assessed at the end of the reproductive season) can vary between 50 oysters per collector to more than 20 000 oysters per collector (Figure 1). This huge variation arise questions from oysters producers and the French National Committee for Shellfish Culture want to get some explanations of the phenomenon. It is well known that...
Tipo: Text Palavras-chave: Larvae; Reproduction; Recruitment; Spatfall; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-4543.pdf
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The Native Oyster Restoration Alliance (NORA) and the Berlin Oyster Recommendation: bringing back a key ecosystem engineer by developing and supporting best practice in Europe ArchiMer
Pogoda, Bernadette; Brown, Janet; Hancock, Boze; Preston, Joanne; Pouvreau, Stephane; Kamermans, Pauline; Sanderson, William; Von Nordheim, Henning.
Efforts to restore the native oyster Ostrea edulis and its associated habitats are gaining momentum across Europe. Several projects are currently running or being planned. To maximize the success of these, it is crucial to draw on existing knowledge and experience in order to design, plan and implement restoration activities in a sustainable and constructive approach. For the development of best practice recommendations and to promote multidimensional knowledge and technology exchange, the Native Oyster Restoration Alliance (NORA) was formed by partners from science, technology, nature conservation, consultancies, commercial producers and policy-makers. The NORA network will enhance scientific and practical progress in flat oyster restoration, such as in...
Tipo: Text Palavras-chave: Ostrea edulis; Berlin Oyster Recommendation; Biogenic reef; Ecosystem service; Biodiversity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00500/61192/64723.pdf
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The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas ArchiMer
Fabioux, Caroline; Pouvreau, Stephane; Leroux, Frederique; Huvet, Arnaud.
The vasa gene is,a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthologue in Crassostrea gigas (Oyvlg). OyVLG contained the eight consensus domains of the DEAD-box including those providing RNA unwinding activity. The expression pattern of Oyvlg was examined in adult oyster tissues at different reproductive stages. Its expression was restricted to germline cells both in males and females, including germinal stem cells and auxiliary cells. The expression of Oyvlg, strongest in early germ cells, decreased as the maturation...
Tipo: Text Palavras-chave: RNA helicase; DEAD box; Vasa; Germline; Gametogenesis; Oyster.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-646.pdf
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Transcriptional regulation of pyruvate kinase and phosphoenolpyruvate carboxykinase in the adductor muscle of the oyster Crassostrea gigas during prolonged hypoxia ArchiMer
Le Moullac, Gilles; Bacca, Helene; Huvet, Arnaud; Moal, Jeanne; Pouvreau, Stephane; Van Wormhoudt, A.
The response of Crassostrea gigas to prolonged hypoxia was investigated for the first time by analyzing the metabolic branch point formed by pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK). PK and PEPCK cDNAs were cloned and sequenced. The main functional domains of the PK sequence, such as the binding sites for ADP/ATP and phosphoenolpyruvate (PEP), were identified whereas the PEPCK sequence showed the specific domain to bind PEP in addition to the kinase-1 and kinase-2 motifs to bind guanosine triphosphate (GTP) and Mg2+, specific for all PEPCKs. A C-terminal extension was detected for the first time in eukaryota PK. Separation of mitochondrial and cytosolic fraction showed that more than 92% of the PEPCK enzyme activity was cytosolic...
Tipo: Text Palavras-chave: Succinate alanine carboxykinase phosphoenolpyruvate pyruvate kinase hypoxia Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2683.pdf
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Trophic environments influence size at metamorphosis and recruitment performance of Pacific oysters ArchiMer
Lagarde, Franck; Richard, Marion; Bec, Beatrice; Roques, Cecile; Mortreux, Serge; Bernard, Ismael; Chiantella, Claude; Messiaen, Gregory; Nadalini, Jean-bruno; Hori, Masakazu; Hamaguchi, Masami; Pouvreau, Stephane; Roque D'Orbcastel, Emmanuelle; Tremblay, Rejean.
Reproduction and recruitment of benthic invertebrates are influenced by the climate and by the ecological structure of marine ecosystems, along with local anthropogenic pressures such as eutrophication or oligotrophication. Using the Pacific oyster Crassostrea gigas as a biological model, we tested the hypothesis that the variability in prodissoconch II (PII) size (i.e. size at metamorphosis) depends on ecological functioning. Settlement and recruitment were assessed at 5 sampling sites on the French Mediterranean shellfish farmed Thau lagoon during the main summer recruitment events in 3 consecutive years (2012-2014). Hydrobiological and planktonic analyses were conducted at 3 sampling sites. Our results showed that recruitment was extremely...
Tipo: Text Palavras-chave: Larval ecology; Recruitment; Prodissoconch II; Crassostrea gigas; Oligotrophication; Cryptophytes; Thau lagoon.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56613/58334.pdf
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Vers un retour de l'huître indigène en rade de Brest ArchiMer
Duchene, Julie; Bernard, Ismael; Pouvreau, Stephane.
Fort appréciée du temps des Romains et très consommée jusqu’au XXème siècle, l’huître plate (Ostrea edulis), huître indigène de nos côtes européennes, ne tient plus aujourd’hui qu’une place anecdotique dans nos assiettes. En France, et plus précisément en Bretagne, des ostréiculteurs et des scientifiques se mobilisent afin d’envisager à nouveau une production durable de cette espèce fortement menacée.
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00270/38085/36220.pdf
Registros recuperados: 115
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